Part:BBa_K5257009

Lpp-OmpT (LOT)

Lpp-OmpT is a small (20 kDa) anchor motif for visualizing proteins on the cell surface. It is a derivative of the E. coli outer membrane protease OmpT fused to the signal peptide and the first nine amino acids of the E. coli lipoprotein Lpp. Hui et al. (2019) demonstrated that it generates highly efficient translocation and stable surface anchoring of the fused gene productions. Unlike OmpA, the LOT system does not generate outer membrane destabilization, which is a great advantage.

The gene sequence was obtained from NCBI https://www.ncbi.nlm.nih.gov/nuccore/MH105774.1/

References

Hui, C. Y., Guo, Y., Liu, L., Zheng, H. Q., Wu, H. M., Zhang, L. Z., & Zhang, W. (2019). Development of a novel bacterial surface display system using truncated OmpT as an anchoring motif. Biotechnology letters, 41, 763-777. https://doi.org/10.1007/s10529-019-02676-4

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]